Detection of Toxoplasma gondii by Polymerase Chain Reaction in Sera of Acutely Infected Mice

J. Hafid; D. Guichard; P. Flori; T. Bourlet; H. Raberin; C. Genin; R. Tran Manh Sung

doi:10.1645/0022-3395(2000)086[0857:DOTGBP]2.0.CO;2

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J. Hafid,¹ D. Guichard,¹ P. Flori,¹ T. Bourlet,¹ H. Raberin,¹ C. Genin,¹ R. Tran Manh Sung¹

¹Groupe immunité des muqueuses et agents pathogènes (GIMAP), Faculté de médecine Jacques Lisfranc, 15 rue Ambroise Paré, 42023 Saint Etienne cedex 02. France

Abstract

The presence of Toxoplasma gondii DNA was detected in sera of acutely infected mice by polymerase chain reaction. Adult mice were inoculated intraperitoneally with 5 × 10³ T. gondii RH strain tachyzoites. Five mice were killed every 3 hr from 3 to 21 hr post infection (PI) and every day from 1 to 7 days PI. Toxoplasma gondii DNA was first detected in 60% of the infected mice 18 hr PI and in 100% of the animals 21 hr PI and from 1 to 7 days PI. No mice survived longer than 7 days.

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J. Hafid, D. Guichard, P. Flori, T. Bourlet, H. Raberin, C. Genin, and R. Tran Manh Sung "Detection of Toxoplasma gondii by Polymerase Chain Reaction in Sera of Acutely Infected Mice," Journal of Parasitology 86(4), 857-859, (1 August 2000). https://doi.org/10.1645/0022-3395(2000)086[0857:DOTGBP]2.0.CO;2

Published: 1 August 2000

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